scSidekick

Reproducible, interpretable single-cell & spatial transcriptomics analysis in R

scSidekick is an open-source R package that takes users from raw count matrices through quality control, integration, clustering, cell-type assignment, differential expression, pathway analysis, and cell–cell communication — while enforcing documentation at every step and producing publication-ready figures, methods text, legends, and PowerPoint exports.

It implements multi-group comparisons, CellChat, inferCNV, cNMF, pseudotime, pseudobulk analysis, ssGSEA, and survival-linked clinical correlation workflows.